Bioanalytical Proteomics and Interactomics - Elisa Michelini

Course aims: The aim of the course is to provide students with basic knowledge and state-of-the-art of analytical techniques involved in structural proteomics, interaction proteomics, protein modification analysis and functional proteomics. At the end of the course, students acquire skills concerning:- underlying principles, merits and limitations of key proteomics analytical techniques, - bioinformatics tools to cope with the analysis of the large amounts of data generated in proteomics experiments, - applications of proteomics in the clinical field and in biomarker discovery
Course contents:   1) ultrasensitive and rapid methods based on molecular recognition combined with selective and highly detectable probes: immunoassays, hybridization reactions, biosensors, FRET,BRET, whole cell biosensors 2) Soft impact mass spectrometry technologies and smart sample handling for proteins /peptides quantitative analysis and identification in complex biological matrices. Combined non invasive separative technologies (FFF) for functional proteomics studies.  3) Luminescence Molecular  imaging: localization and quantification of analytes in single cells and tissues (combined with microscopy), whole organ and living organisms using nanoparticles and recombinant bioluminescent cells.

Readings/Bibliography: on line, selected papers and books.
- Introduction to Proteomics: Principles and Applications (Methods of Biochemical Analysis). Nawin C. Mishra, Günter Blobel, 2010. Wiley
- LC-MS/MS in Proteomics: Methods and Applications (Methods in Molecular Biology). Pedro R. Cutillas, John F. Timms, 2010. Humana press

Teaching methods: The course consists both in lectures dealing about the topics listed in the program and a series of laboratory experiments and/or demonstrations concerning common proteomics procedures and applications.
Assessment methods: Oral examination
Teaching tools: Video and overhead projector. Slides of all the lectures are available as PowerPoint files.